Antisense technology+research paper

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Antisense technology+research paper

Antisense technology offers almost unlimited scope for the development of new methods of drug design. It is one of the most approved approaches among several others, for inactivating a single chosen gene. It has wide applications in crop improvement, treatment of genetic disorders, viral resistance, gene regulation etc The complementary strand to this strand is called an antisense.

How Antisense Therapy Works

The antisense technology works by binding of antisense strand with the targeted sense strand through hydrogen bond. The double stranded molecule will be recognized as a foreign molecule and is susceptible to degradation. Though, as the DNA is double helix, it can be used in Antisense technology+research paper antisense technology; resulting in the formation of triplex.

Antisense strand can be: The catalytic antisense strands are called ribozymes, which cuts the RNA molecules at specific position while the non catalytic antisense strand blocks the processing of RNA. More widely used than DNA Stages of inhibition of antisense Antisense strand can act at three stages in cells 1 Transcription is the first stage at which a target gene can be blocked.

But there is no proper evidence for this. When antisense strand binds with the sense strand, there is a formation of duplex. But there is no direct evidence for duplex formation perhaps due to quick disintegration of double stranded RNA mRNA-antisense hybridthus making the sense or target mRNA unavailable for translation.

Antisense strand competes with the ribosomes for the binding site of sense RNA, thus inhibiting the translation. Once the ribosome binds the mRNA, it will translate to form functional protein.

So, for the significant inhibition of target mRNA, antisense strand must bind to it before ribosome. Mechanism Antisense RNA is constructed by reversing the orientation of a gene with regard to its promoter so that the antisense strand is transcribed e. When an antisense strand binds to the targeted sense strand, double helix forms and cell will recognize this double helix as a foreign molecule and work in a direction to destroy it.

Binding of antisense strand to their target sense strand activates a cellular enzyme called RNase H. This enzyme degrades the target RNA, thus inhibits the formation of a particular protein.

On the basis of mechanism of regulation of antisense RNA, they are of 3 types: Applications of antisense technology 1 Artificial antisense RNA regulation of gene expression Antisense RNA has also been used to artificially modulate gene expression in plants and animals.

Antisense technology+research paper

To determine the optimum requirement for an efficient antisense RNA regulation, varied lengths as well as regions of sense gene of B-galactosidase were targeted. This experiment leads to the following conclusions: This involves the inhibition of polygalacturonase PG gene, which codes for the polygalacturonase enzyme.

This enzyme causes the degradation of cell wall that result in fruit ripening. Inhibition of PG gene expression greatly reduces the fruit ripening, thus improves shelf life of tomatoes. Antisense technology has also been applied for potato crop to reduce the discoloration of tubers after bruising.The neutralization of antiapoptotic BCL-2 family proteins by antisense technology or by overexpression of proapoptotic BCL-2 family proteins has remained an active area of apoptosis research .

Antisense technology offers almost unlimited scope for the development of new methods of drug design. It is one of the most approved approaches among several others, for inactivating a single chosen gene. It has wide applications in crop improvement, treatment of genetic disorders, viral resistance, gene regulation etc.

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CMT1A Research Breakthrough! (CMTA), have identified a promising early-stage therapeutic approach with antisense technology for CMT Type 1A (CMT1A), the most common form of the disease, accounting for half of all cases.

There is currently no treatment or cure for CMT, making the CMT1A research a significant and hopeful . Gudkov Roswell Park Cancer Institute, Buffalo, NY.

Technology

Daniele Silvestro and Ingo Michalak of the Department of Botany and Molecular Evolution of the Senckenberg Research Institute, antisense technology research paper Frankfurt am Main, Germany (raxmlgui.

CMT1A Ionis Press Release. (CMTA), have identified a promising early-stage therapeutic approach with antisense technology for CMT Type 1A (CMT1A), the most common form of the disease, accounting for half of all cases. For more information about the research paper, please go to iridis-photo-restoration.com

Antisense Technology - an overview | ScienceDirect Topics